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The workflow of treatment in pancreatic cancer in mice.

Journal: Acta Cirúrgica Brasileira

Article Title: Hypofractionated radiotherapy followed by rhGM-CSF enhances immunogenic cell death in a murine model of pancreatic cancer

doi: 10.1590/acb407625

Figure Lengend Snippet: The workflow of treatment in pancreatic cancer in mice.

Article Snippet: The tumor sections were washed three times with PBS and then incubated in 3% peroxide for 3 min, followed by incubation in 5% bovine serum albumin (BSA) at room temperature for 30 min. Primary antibodies against hypoxia-inducible factor-1α (HIF-1α) (20960-1-AP, Proteintech), hypoxia-inducible factor-2α (HIF-2α) (PA1-16510, Thermo Fisher Scientific), soluble vascular endothelial growth factor (VEGF) receptor-1 (sVEGFR-1) (36-1100, Thermo Fisher Scientific), and VEGF (19003-1-AP, Proteintech) were used to incubate with tumor sections overnight at 4°C.

Techniques:

rhGM-CSF combined with HFRT inhibits the angiogenesis-related factors expression in PC tumors. Tumors were treated with rhGM-CSF and HFRT, respectively, or in combination (HFRT followed by rhGM-CSF treatment, HFRT & rhGM-CSF treatment concurrently, and rhGM-CSF treatment followed by HFRT). ( a and e ) HIF-1α, ( b and f ) HIF-2α, ( c and g ) sVEGFR-1, ( d and h ) VEGF were measured by immunohistochemistry assay.

Journal: Acta Cirúrgica Brasileira

Article Title: Hypofractionated radiotherapy followed by rhGM-CSF enhances immunogenic cell death in a murine model of pancreatic cancer

doi: 10.1590/acb407625

Figure Lengend Snippet: rhGM-CSF combined with HFRT inhibits the angiogenesis-related factors expression in PC tumors. Tumors were treated with rhGM-CSF and HFRT, respectively, or in combination (HFRT followed by rhGM-CSF treatment, HFRT & rhGM-CSF treatment concurrently, and rhGM-CSF treatment followed by HFRT). ( a and e ) HIF-1α, ( b and f ) HIF-2α, ( c and g ) sVEGFR-1, ( d and h ) VEGF were measured by immunohistochemistry assay.

Article Snippet: The tumor sections were washed three times with PBS and then incubated in 3% peroxide for 3 min, followed by incubation in 5% bovine serum albumin (BSA) at room temperature for 30 min. Primary antibodies against hypoxia-inducible factor-1α (HIF-1α) (20960-1-AP, Proteintech), hypoxia-inducible factor-2α (HIF-2α) (PA1-16510, Thermo Fisher Scientific), soluble vascular endothelial growth factor (VEGF) receptor-1 (sVEGFR-1) (36-1100, Thermo Fisher Scientific), and VEGF (19003-1-AP, Proteintech) were used to incubate with tumor sections overnight at 4°C.

Techniques: Expressing, Immunohistochemistry